Journal: PLoS ONE

Article Title: CXCR4 expression in lung carcinogenesis: Evaluating gender-specific differences in survival outcomes based on CXCR4 expression in early stage non-small cell lung cancer patients

doi: 10.1371/journal.pone.0241240

Figure Lengend Snippet: Frequency and percent of patient and tumor characteristics at diagnosis for all patients and by gender.

Article Snippet: Briefly, 5 μm sections were cut from TMA blocks and stained using a 1:500 dilution of anti-pan-cytokeratin mouse monoclonal antibody (Dako) and a 1:25 dilution of an anti-CXCR4 rabbit monoclonal antibody (clone UMB2, Biotrend, Köln, Germany) to identify tumor cells and CXCR4 expression, respectively [ ].

Techniques: Biomarker Discovery

Journal: PLoS ONE

Article Title: CXCR4 expression in lung carcinogenesis: Evaluating gender-specific differences in survival outcomes based on CXCR4 expression in early stage non-small cell lung cancer patients

doi: 10.1371/journal.pone.0241240

Figure Lengend Snippet: CXCR4 expression in negative and positive (HeLa) control cells (left panel). CXCR4 staining in early stage non-small cell lung cancer patients, with low (right top panel) and high CXCR4 expression (right bottom panel) shown in red. DAPI nuclear staining shown in blue, and PCK tumor cytosolic component shown in green.

Article Snippet: Briefly, 5 μm sections were cut from TMA blocks and stained using a 1:500 dilution of anti-pan-cytokeratin mouse monoclonal antibody (Dako) and a 1:25 dilution of an anti-CXCR4 rabbit monoclonal antibody (clone UMB2, Biotrend, Köln, Germany) to identify tumor cells and CXCR4 expression, respectively [ ].

Techniques: Expressing, Control, Staining

Journal: PLoS ONE

Article Title: CXCR4 expression in lung carcinogenesis: Evaluating gender-specific differences in survival outcomes based on CXCR4 expression in early stage non-small cell lung cancer patients

doi: 10.1371/journal.pone.0241240

Figure Lengend Snippet: Mean CXCR4 Max expression increased with stage progression, with higher CXCR4 expression in stage IV NSCLC patients (top panel); error bars represent standard error of the mean. Maximum CXCR4 expression levels >2500 were less frequent in early stage lung cancer patients compared to late stage patients (bottom panel).

Article Snippet: Briefly, 5 μm sections were cut from TMA blocks and stained using a 1:500 dilution of anti-pan-cytokeratin mouse monoclonal antibody (Dako) and a 1:25 dilution of an anti-CXCR4 rabbit monoclonal antibody (clone UMB2, Biotrend, Köln, Germany) to identify tumor cells and CXCR4 expression, respectively [ ].

Techniques: Expressing

Journal: PLoS ONE

Article Title: CXCR4 expression in lung carcinogenesis: Evaluating gender-specific differences in survival outcomes based on CXCR4 expression in early stage non-small cell lung cancer patients

doi: 10.1371/journal.pone.0241240

Figure Lengend Snippet: There was an increase in both the nuclear and cytomembranous (cyto) CXCR4 expression in the late stage (stage IV) sample compared to the early stage sample in the female patient (grey bars). Conversely, there was no difference in CXCR4 expression in the male patient, irrespective of stage (black bars).

Article Snippet: Briefly, 5 μm sections were cut from TMA blocks and stained using a 1:500 dilution of anti-pan-cytokeratin mouse monoclonal antibody (Dako) and a 1:25 dilution of an anti-CXCR4 rabbit monoclonal antibody (clone UMB2, Biotrend, Köln, Germany) to identify tumor cells and CXCR4 expression, respectively [ ].

Techniques: Expressing

Journal: PLoS ONE

Article Title: CXCR4 expression in lung carcinogenesis: Evaluating gender-specific differences in survival outcomes based on CXCR4 expression in early stage non-small cell lung cancer patients

doi: 10.1371/journal.pone.0241240

Figure Lengend Snippet: Impact of CXCR4 expression on recurrence, RFS and OS.

Article Snippet: Briefly, 5 μm sections were cut from TMA blocks and stained using a 1:500 dilution of anti-pan-cytokeratin mouse monoclonal antibody (Dako) and a 1:25 dilution of an anti-CXCR4 rabbit monoclonal antibody (clone UMB2, Biotrend, Köln, Germany) to identify tumor cells and CXCR4 expression, respectively [ ].

Techniques: Expressing

Journal: PLoS ONE

Article Title: CXCR4 expression in lung carcinogenesis: Evaluating gender-specific differences in survival outcomes based on CXCR4 expression in early stage non-small cell lung cancer patients

doi: 10.1371/journal.pone.0241240

Figure Lengend Snippet: There was no difference in recurrence-free survival (top panel) or overall survival (bottom panel) based on gender in patients with high or low levels of CXCR4 expression based on median splits.

Article Snippet: Briefly, 5 μm sections were cut from TMA blocks and stained using a 1:500 dilution of anti-pan-cytokeratin mouse monoclonal antibody (Dako) and a 1:25 dilution of an anti-CXCR4 rabbit monoclonal antibody (clone UMB2, Biotrend, Köln, Germany) to identify tumor cells and CXCR4 expression, respectively [ ].

Techniques: Expressing

Journal: Narra J

Article Title: Estimate the relationship between CXCR4-SDF-1 axis and inhibitory molecules (CTLA4 and PD-1) in patients with colon cancer

doi: 10.52225/narra.v4i3.992

Figure Lengend Snippet: Comparison of serum level of immunological parameters (CXCR4 and SDF-1) between colon cancer and control groups

Article Snippet: The levels of CXCR4 and SDF-1 in sera were measured using sandwich enzyme-linked immune-sorbent assay (ELISA) with Human CXC-chemokine receptor 4 (CXCR4) ELISA Kit and Human Stromal cell-derived factor 1 (SDF-1) ELISA kit, respectively (both from YL Biotech, Shanghai, China).

Techniques: Comparison, Control

Journal: Narra J

Article Title: Estimate the relationship between CXCR4-SDF-1 axis and inhibitory molecules (CTLA4 and PD-1) in patients with colon cancer

doi: 10.52225/narra.v4i3.992

Figure Lengend Snippet: Comparisons of CXCR4 and SDF-1 levels across age, sex, colon cancer grades, and stages

Article Snippet: The levels of CXCR4 and SDF-1 in sera were measured using sandwich enzyme-linked immune-sorbent assay (ELISA) with Human CXC-chemokine receptor 4 (CXCR4) ELISA Kit and Human Stromal cell-derived factor 1 (SDF-1) ELISA kit, respectively (both from YL Biotech, Shanghai, China).

Techniques: Control

Journal: Narra J

Article Title: Estimate the relationship between CXCR4-SDF-1 axis and inhibitory molecules (CTLA4 and PD-1) in patients with colon cancer

doi: 10.52225/narra.v4i3.992

Figure Lengend Snippet: Correlations between cytokines and inhibitory molecules

Article Snippet: The levels of CXCR4 and SDF-1 in sera were measured using sandwich enzyme-linked immune-sorbent assay (ELISA) with Human CXC-chemokine receptor 4 (CXCR4) ELISA Kit and Human Stromal cell-derived factor 1 (SDF-1) ELISA kit, respectively (both from YL Biotech, Shanghai, China).

Techniques:

Journal: International Journal of Nanomedicine

Article Title: Mesenchymal Stem Cell Derived Exosomes as Nanodrug Carrier of Doxorubicin for Targeted Osteosarcoma Therapy via SDF1-CXCR4 Axis

doi: 10.2147/IJN.S372851

Figure Lengend Snippet: The expression of CXCR4, SDF1 and tubulin proteins in 143B, MG63, HOS, H9C2, BM-MSCs cells and purified exosome measured by Western-blot analysis.

Article Snippet: Antibodies against CXCR4, SDF1 and Tubulin were obtained from Affinity Biosciences (1:1000; Cincinnati, USA) and the negative marker calnexin was obtained from Thermo Scientific (Waltham, MA, USA).

Techniques: Expressing, Purification, Western Blot

Journal: Oncology Letters

Article Title: 786-0 Renal cancer cell line-derived exosomes promote 786-0 cell migration and invasion in vitro

doi: 10.3892/ol.2014.1962

Figure Lengend Snippet: 786-0 cell-derived exosomes increase CXCR4 and MMP-9 expression. 786-0 cells were pretreated with Exo (100 μg/ml), PBS (7 mmol/l) and Amiloride, 5-(N,N-Dimethyl)-, hydrochloride (7 mmol/l; Exo-D) for 24 h. Total protein was extracted for western blot analysis. CXCR4 and MMP-9 expression were significantly increased in the Exo-treated group compared with the Exo-D and PBS-treated groups (P<0.05). Exo, exosomes; PBS, phosphate-buffered saline; Exo-D, exosomes depression; CXCR4, chemokine receptor type 4; MMP-9, matrix metalloproteinase-9.

Article Snippet: The membranes were blocked with 5% skimmed milk in Tris-buffered saline containing 0.1% Tween-20 for 1 h and incubated with primary antibodies (1:200 dilution) against human chemokine receptor type 4 (CXCR4) and matrix metalloproteinase-9 (MMP-9) obtained from Tianjin Saier Biotechnology Co., Ltd. (Tianjin, China) overnight at 4°C.

Techniques: Derivative Assay, Expressing, Western Blot, Saline

Journal: Asia Pacific Allergy

Article Title: Identification and validation of autophagy-related genes and exploration of their relationship with disease severity in chronic rhinosinusitis with nasal polyps

doi: 10.5415/apallergy.0000000000000159

Figure Lengend Snippet: qRT-PCR and immunohistochemistry staining validate the expression levels of the central genes in clinical samples. (A–E) The mRNA expression levels of CXCR4, TNFSF11, HMOX1, APOE , and SPP1 . (F) Representative immunohistochemistry staining of CXCR4, HMOX1 , and SPP1 . (G–I) Quantitative summary of the mean intensity of CXCR4, HMOX1 , and SPP1 . qRT-PCR (control group: n=21; E-CRSwNP group: n=9; NE-CRSwNP group: n=12). Immunohistochemistry staining (control group: n=3; E-CRSwNP group: n=3; NE-CRSwNP group: n=3). Images were captured at ×400 magnification. Data are shown as means ± SEM. E-CRSwNP, eosinophilic chronic rhinosinusitis with nasal polyps; IOD, integrated optical density; NE-CRSwNP, noneosinophilic chronic rhinosinusitis with nasal polyps; qRT-PCR, quantitative real-time polymerase chain reaction. *P <0.05, **P <0.01, ****P <0.0001. ns, P >0.05.

Article Snippet: Subsequently, the sections were subjected to incubation with primary antibodies against CXCR4 (1:100; Wanleibio, Shenyang, China), HMOX1 (1:150; Wanleibio), and SPP1 (1:100; Wanleibio) at a temperature of 4 °C for the duration of the overnight period.

Techniques: Quantitative RT-PCR, Immunohistochemistry, Staining, Expressing, Control, Real-time Polymerase Chain Reaction

Journal: Asia Pacific Allergy

Article Title: Identification and validation of autophagy-related genes and exploration of their relationship with disease severity in chronic rhinosinusitis with nasal polyps

doi: 10.5415/apallergy.0000000000000159

Figure Lengend Snippet: Western blotting analysis and correlation analysis between the central genes and LC3B in CRSwNP. (A–C) Protein expression levels of CXCR4, HMOX1 , and SPP1 were assayed by western blot (control group: n=4; E-CRSwNP group: n=4; NE-CRSwNP group: n=4). (D) The mRNA expression level of LC3B was assayed by qRT-PCR. (E) Protein expression levels of LC3B were assayed by western blot (control group: n=5; E-CRSwNP group: n=4; NE-CRSwNP group: n=4). (F–H) The correlation between CXCR4, HMOX1, SPP1 , and LC3B. The Pearson correlation test was used. qRT-PCR (control group: n=21; E-CRSwNP group: n=9; NE-CRSwNP group: n=12). Data are shown as means ± SEM. CRSwNP, chronic rhinosinusitis with nasal polyps; E-CRSwNP, eosinophilic chronic rhinosinusitis with nasal polyps; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; NE-CRSwNP, noneosinophilic chronic rhinosinusitis with nasal polyps; qRT-PCR, quantitative real-time polymerase chain reaction. *P <0.05, **P <0.01, ***P <0.001. ns, P >0.05.

Article Snippet: Subsequently, the sections were subjected to incubation with primary antibodies against CXCR4 (1:100; Wanleibio, Shenyang, China), HMOX1 (1:150; Wanleibio), and SPP1 (1:100; Wanleibio) at a temperature of 4 °C for the duration of the overnight period.

Techniques: Western Blot, Expressing, Control, Quantitative RT-PCR, Real-time Polymerase Chain Reaction

Journal: Asia Pacific Allergy

Article Title: Identification and validation of autophagy-related genes and exploration of their relationship with disease severity in chronic rhinosinusitis with nasal polyps

doi: 10.5415/apallergy.0000000000000159

Figure Lengend Snippet: The correlation between CXCR4, HMOX1 , and SPP1 and the disease severity parameters. The Spearman rank test was used. CT, computed tomography; NE, neutrophil elastase; SNOT-22, The Sino-Nasal Outcome Test 22.

Article Snippet: Subsequently, the sections were subjected to incubation with primary antibodies against CXCR4 (1:100; Wanleibio, Shenyang, China), HMOX1 (1:150; Wanleibio), and SPP1 (1:100; Wanleibio) at a temperature of 4 °C for the duration of the overnight period.

Techniques: Computed Tomography